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ice cold tbs ip buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ice cold tbs ip buffer
    Ice Cold Tbs Ip Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ice+cold+chaps+ip+buffer/CHAPS/pmc09007588-316-18-35
    Average 97 stars, based on 1 article reviews
    ice cold tbs ip buffer - by Bioz Stars, 2026-11
    97/100 stars

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    other:

    Article Title: The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1
    Article Snippet: Protein A/G-Sepharose beads were added to the samples, centrifuged, washed either in ice-cold CHAPS IP buffer (BAK/BCLXL and BAX/BCLXL IPs; 0.3% CHAPS, 40 m m Hepes, pH 7.5, 120 m m NaCl, 1 m m EDTA, 10 m m pyrophosphate and protease/phosphatase inhibitors (Pierce)), or RIPA IP buffer (p300/ETS1 IPs; 150 m m NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 m m Tris) and resuspended in Laemmli sample buffer.

    Article Title: The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1
    Article Snippet: Protein A/G sepharose beads were added to the samples, centrifuged, washed either in ice-cold CHAPS IP buffer (BAK/BCLXL and BAX/BCLXL IPs; 0.3% CHAPS, 40 mM Hepes, pH 7.5, 120 mM NaCl, 1 mM EDTA, 10 mM pyrophosphate and protease/phosphatase inhibitors [Pierce]), or RIPA IP buffer (p300/ETS1 IPs; 150mM NaCl, 1% nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 25mM Tris) and resuspended in Laemmli sample buffer.

    Article Title: The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1
    Article Snippet: For block PCR, a GeneAmp PCR System 9700 (PE Applied Biosystems) was utilized and the resulting amplicons were resolved on a 1.5% agarose gel stained with ethidium bromide.



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